<i>In vivo</i> and <i>in vitro</i> evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan <i>Punica granatum</i> L.

Introduction: The use of pomegranates in Moroccan pharmacopeia is due to their healing and nutritional properties because richness secondary molecules. following study analyses the composition aqueous extract Punica granatum peel evaluates vivo vitro antioxidant effects, hemolytic protection, acute toxicity. Methods: Quantification plant was realized by high-performance liquid chromatography (HPLC). assay used for erythrocyte while effect evaluated 2, 20-azinobis-(3-ethylbenzothiazoneline-6-sulphonic acid) (ABTS) reducing ferric power (RFP) assays. in-vivo activity tested measuring levels lipid peroxidation (LPO) serum. toxicological oral administration four groups mice 21 days, followed a histopathological examination spleen. Results: HPLC analysis showed presence some phenolic compounds such as coumarin, caffeic, gallic syringic acids. IC50 assays were 254.49 ± 62.17 μg/mL 40.265 2.9 ABTS power, respectively. Furthermore, thiobarbituric acid reactive substances (TBARS) lowest at 150 mg/mL concentration. All concentrations protection did not exceed 15% hemolysis. Moreover, toxicity test no sign mortality, signs weakness, or weight loss; also spleen tissues absence any damage. Conclusion: P. good potential could be exploited natural antihemolytic remedy, leading development new drugs.